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Your Position: Home > Protein > Mesothelin > MSN-H826x

Biotinylated Human Mesothelin / MSLN (296-580) Protein, Fc Tag, ultra sensitivity (primary amine labeling) (MALS verified)

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  • Synonym
    MSLN,Mesothelin,MPF
  • Source
    Biotinylated Human Mesothelin (296-580) Protein, Fc Tag, primary amine labeling(MSN-H826x) is expressed from human 293 cells (HEK293). It contains AA Glu 296 - Gly 580 (Accession # AAH09272 ).
  • Molecular Characterization
    Mesothelin Structure

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 59.1 kDa. The protein migrates as 60-68 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mesothelin SDS-PAGE

Biotinylated Human Mesothelin (296-580) Protein, Fc Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Mesothelin MALS images

The purity of Biotinylated Human Mesothelin (296-580) Protein, Fc Tag, primary amine labeling (Cat. No. MSN-H826x) is more than 90% and the molecular weight of this protein is around 125-153 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Mesothelin ELISA

Immobilized Anti-Human MSLN Mab at 0.5 μg/mL (100 μL/well) can bind Biotinylated Human Mesothelin (296-580) Protein, Fc Tag, primary amine labeling (Cat. No. MSN-H826x) with a linear range of 0.5-8 ng/mL (QC tested).

Evaluation of CAR expression
FACS Analysis of Anti-MSLN CAR Expression
 Mesothelin CAR_T

293 cells were transfected with anti-MSLN-scFv and RFP tag. 2e5 of the cells were first stained with B. Biotinylated Human Mesothelin (296-580) Protein, Fc Tag, primary amine labeling (Cat. No. MSN-H826x, 3 µg/ml) and C. Biotinylated Protein Control, followed by FITC Streptavidin. A. Non-transfected 293 cells and C. Biotinylated Protein Control were used as negative control. RFP was used to evaluate CAR (anti-MSLN-scFv) expression and FITC was used to evaluate the binding activity of Biotinylated Human Mesothelin (296-580) Protein, Fc Tag, primary amine labeling (Cat. No. MSN-H826x).

  • Background
    Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.
  • Clinical and Translational Updates

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