Human CD155 / PVR Protein, Mouse IgG2a Fc Tag, low endotoxin

INTRODUCTION

QC & Assay Protocol

Citation & Background

Synonym
PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5
Source
Human CD155, mouse IgG2a Fc Tag (CD5-H5254) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Asn 343 (Accession # NP_006496.2).
Predicted N-terminus: Trp 21
Request for sequence
Molecular Characterization
This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 62.2 kDa. As a result of glycosylation, the protein migrates as 70-100 kDa under reducing (R) condition, and 130-160 kDa under non-reducing (NR) condition (SDS-PAGE).
Endotoxin
Less than 0.1 EU per μg by the LAL method.
Purity
>95% as determined by SDS-PAGE.

Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss was observed after storage at:
1. 4-8^°C for 12 months in lyophilized state;
2. -70^°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE

Human CD155, mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA

Human CD155, mouse IgG2a Fc TagHuman CD155, mouse IgG2a Fc Tag (Cat. No. CD5-H5254) ELISA bioactivity

Immobilized Human TIGIT, Fc Tag (Cat. No. TIT-H5254) at 2μg/mL (100 μL/well) can bind Human CD155, mouse IgG2a Fc tag (Cat. No. CD5-H5254) with a linear range of 0.98-7.81 ng/mL (QC tested).

Request for protocol

Immobilized Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) at 2μg/mL (100 μL/well) can bind Human CD155, mouse IgG2a Fc tag (Cat. No. CD5-H5254) with a linear range of 0.98-15.63 ng/mL (Routinely tested).

Request for protocol

Bioactivity-FACS

FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that recombinant Human CD155, mouse IgG2a Fc Tag (Cat. No. CD5-H5254) can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 μg/ml (Routinely tested).

Request for protocol

FACS analysis shows that the binding of Human CD155, mouse IgG2a Fc Tag to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 μg/ml. The IC50 is 0.1413 μg/ml (Routinely tested).

Request for protocol

Background
CD155 (cluster of differentiation 155) also known as the poliovirus receptor is a protein that is encoded by the PVR gene. CD155 is a Type I transmembrane glycoprotein in the immunoglobulin superfamily. Commonly known as Poliovirus Receptor (PVR) due to its involvement in the cellular poliovirus infection in primates, CD155's normal cellular function is in the establishment of intercellular adherens junctions between epithelial cells. The role of CD155 in the immune system is unclear, though it may be involved in intestinal humoral immune responses. Subsequent data has also suggested that CD155 may also be used to positively select MHC-independent T cells in the thymus.
References
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