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Your Position: Home > Immune Checkpoint Proteins > CD155 > Human CD155 / PVR Protein, Mouse IgG2a Fc Tag, low endotoxin

Human CD155 / PVR Protein, Mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    PVR,FLJ25946,PVS,CD155,TAGE4,HVED,NECL5
  • Source
    Human CD155, Mouse IgG2a Fc Tag, low endotoxin (CD5-H5254) is expressed from human 293 cells (HEK293). It contains AA Trp 21 - Asn 343 (Accession # NP_006496.2).
    Predicted N-terminus: Trp 21
  • Molecular Characterization
    Online(Trp 21 - Asn 343) NP_006496.2

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 62.2 kDa. As a result of glycosylation, the protein migrates as 70-100 kDa under reducing (R) condition, and 130-160 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) SDS-PAGE gel

Human CD155, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human CD155, Mouse IgG2a Fc Tag, low endotoxinHuman CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) ELISA bioactivity

Immobilized Human TIGIT, Fc Tag (Cat. No. TIT-H5254) at 2 μg/mL (100 μL/well) can bind Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) with a linear range of 0.1-4 ng/mL (QC tested).

Human CD155, Mouse IgG2a Fc Tag, low endotoxinHuman CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) ELISA bioactivity

Immobilized Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) at 2 μg/mL (100 μL/well) can bind Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) with a linear range of 1-16 ng/mL (Routinely tested).

Human CD155, Mouse IgG2a Fc Tag, low endotoxinHuman CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) ELISA bioactivity

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254): Biotinylated Human TIGIT, Fc,Avitag (Cat. No. TIT-H82F1) binding reactions. The half maximal inhibitory concentration (IC50) is 0.08116 μg/mL (Routinely tested).

Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

FACS assay shows that Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) can bind to 293T cell overexpressing human TIGIT. The concentration of CD155 is 1 μg/mL (Routinely tested).

FACS assay shows that recombinant Biotinylated FACS

FACS analysis shows that the binding of Human CD155, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CD5-H5254) to 293T overexpressing TIGIT was inhibited by increasing concentration of neutralizing Anti-Human TIGIT MAb. The concentration of CD155 used is 1 μg/mL. The IC50 is 0.1413 μg/mL (Routinely tested).

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