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Your Position: Home > All Other Proteins > BCMA > BCA-H5253

Human BCMA / TNFRSF17 Protein, Mouse IgG2a Fc Tag, low endotoxin

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    TNFRSF17,CD269,BCM,BCMA
  • Source
    Human BCMA, Mouse IgG2a Fc Tag, low endotoxin (BCA-H5253) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 54 (Accession # Q02223-1).
    Predicted N-terminus: Met 1
    Request for sequence
  • Molecular Characterization
    Online(Met 1 - Ala 54) Q02223-1

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 32.8 kDa. The protein migrates as 35-42 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) SDS-PAGE gel

Human BCMA, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human BCMA, Mouse IgG2a Fc TagHuman BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) ELISA bioactivity

Immobilized Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) at 1 μg/mL (100 μL/well) can bind Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) with a linear range of 0.2-6 ng/mL (QC tested).

Protocol
Human BCMA, Mouse IgG2a Fc TagHuman BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) ELISA bioactivity

Immobilized Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) at 2 μg/mL (100 μL/well) can bind Anti-Human BCMA MAb with a linear range of 0.2-3 ng/mL (Routinely tested).

Protocol
Human BCMA, Mouse IgG2a Fc TagHuman BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) ELISA bioactivity

Immobilized Human APRIL, Fc Tag (Cat. No. APL-H5267) at 5 μg/mL (100 μL/well) can bind Human BCMA, Mouse IgG2a Fc Tag (Cat. No. BCA-H5253) with a linear range of 0.2-2 ng/mL (Routinely tested).

Protocol

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