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Your Position: Home > All Other Proteins > Nectin-2 > Human Nectin-2 / CD112 Protein, Mouse IgG2a Fc Tag

Human Nectin-2 / CD112 Protein, Mouse IgG2a Fc Tag

  • Synonym
    CD112,Nectin-2,PVRL2
  • Source
    Human Nectin-2, Mouse IgG2a Fc Tag (CD2-H5257) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Leu 360 (Accession # Q92692-2).
    Predicted N-terminus: Gln 32
  • Molecular Characterization
    Online(Gln 32 - Leu 360) Q92692-2

    This protein carries a mouse IgG2a Fc tag at the C-terminus.

    The protein has a calculated MW of 62.4 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) SDS-PAGE gel

Human Nectin-2, Mouse IgG2a Fc Tag on SDS-PAGE  under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Nectin-2, Mouse IgG2a Fc TagHuman Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) ELISA bioactivity

Immobilized Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) at 5 μg/mL (100 μL/well) can bind Human Nectin-2, Mouse IgG2a Fc Tag (Cat. No. CD2-H5257) with a linear range of 2-20 ng/mL (QC tested).

  • Background
    Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB),  which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Price(USD) : $1980.00

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