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Your Position: Home > Protein > PLAU > PLU-H5228

Human PLAU / uPA Protein, His Tag (activated by trypsin) (active enzyme)

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  • Synonym
    Urokinase,PLAU,ATF,UPA,URK,u-PA,BDPLT5,QPD
  • Source
    Human PLAU, His Tag (activated by trypsin)(PLU-H5228) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Leu 431  (Accession # P00749-1 ).
    Predicted N-terminus: Ser 21 & Lys156 & Ile179
  • Molecular Characterization
    PLAU Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The active form of Human PLAU is a disulfide-linked heterodimer composed of long chain A (Ser 21 - Phe 177) and chain B (Ile 179 - Leu 431) with calculated MW of 17.8 kDa and 29.2 kDa. The long chain A is further cleaved to yield a short chain A (Lys 156 - Phe 177) and N-terminal fragment (Ser 21 - Lys 155) with calculated MW of 15.3 kDa. The protein migrates as 17 kDa (N-terminal fragment), 32-35 kDa (chain B) and 45-50 kDa (long chain A & chain B) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 25 mM HEPES, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
PLAU SDS-PAGE

Human PLAU, His Tag (activated by trypsin) on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity

Measured by it's ability to cleave a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The specific activity is >2,000 pmol/min/µg, as measured under the described conditions (QC tested).

  • Background
    Urokinase - type plasminogen activator is also known as PLAU and UPA, a serine protease with an extremely limited substrate specificity, cleaving the sequence Cys – Pro – Gly - Arg560 - Val561 – Val – Gly – Gly – Cys in plasminogen to form plasmin. uPA is a potent marker of invasion and metastasis in a variety of human cancers associated with breast, stomach, colon, bladder, ovary, brain and endometrium.uPA and its receptor (uPAR) have been implicated in a broad spectrum of pathophysiological processes, including fibrinolysis, proteolysis, inflammation, atherogenesis and plaque destabilization, all of which are involved in the pathogenesis of MI (myocardial infarction).
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:4 Details
  • Number of Drugs in Clinical Trials:5 Details
  • Latest Research Phase:Approved

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