Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.
The protein has a calculated MW of 116.3 kDa. The protein migrates as 110-120 kDa under reducing (R) condition (SDS-PAGE).
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human FGL1 (64-312), Avitag,Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Loaded Biotinylated Human FGL1 (64-312), Avitag,Fc Tag (Cat. No. FG1-H82F6) on SA Biosensor, can bind Human LAG-3, Fc Tag (HPLC-verified) (Cat. No. LA3-H5255) with an affinity constant of 3.78 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
FACS assay shows that Biotinylated Human FGL1 (64-312), Avitag,Fc Tag (Cat. No. FG1-H82F6) can bind to 293T cells overexpressing human LAG3. The concentration of Human FGL1 is 3 μg/ml (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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