Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a human IgG1 Fc tag.
The protein has a calculated MW of 51.9 kDa. The protein migrates as 65-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human CLEC12A, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Biotinylated Human CLEC12A, Fc,Avitag (Cat. No. CLA-H82F7) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human CLEC12A Antibody, Human IgG1 with a linear range of 0.1-2 ng/mL (QC tested).
2e5 of anti-CLEC12A CAR-293 were stained with 100 μL of 3 μg/mL of Biotinylated Human CLEC12A, Fc,Avitag (Cat. No. CLA-H82F7) and negative control protein respectively, washed and then followed by PE-SA and analyzed with FACS (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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