This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 58.8 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>85% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human LGR4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 85%.
Human R-Spondin 3 immobilized on CM5 Chip can bind Human LGR4, His Tag (Cat. No. LG4-H52H3) with an affinity constant of 0.394 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
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