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Your Position: Home > All Other Proteins > IDH1 > ID1-H51H6

Human IDH1 Protein, His Tag (active enzyme, MALS verified)

  • Synonym
  • Source
    Human IDH1, His Tag (ID1-H51H6) is expressed from E.coli cells. It contains AA Met 1 - Leu 414 (Accession # O75874-1).
  • Molecular Characterization
    Online(Met 1 - Leu 414) O75874-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 48.5 kDa. The protein migrates as 45-48 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • Shipping
    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
Human IDH1, His Tag (Cat. No. ID1-H51H6) SDS-PAGE gel

Human IDH1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Human IDH1, His Tag (Cat. No. ) MALS images

The purity of Human IDH1, His Tag (Cat. No. ID1-H51H6) was more than 85% and the molecular weight of this protein is around 90-100 kDa verified by SEC-MALS.


Measured by the ability to oxidatively decarboxylate isocitrate to 2-oxoglutarate. The specific activity is >10000 pmol/min/μg, as measured under the described conditions (QC tested).

  • Background
    The IDH enzymes catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (α-KG), producing nicotinamide adenine dinucleotide phosphate (NADPH) in the process via the citric acid cycle. Eukaryotic cells express two distinct classes of IDHs that utilize either NAD or NADP as their cofactors and serve diverse biological functions. NAD-dependent IDH is localized to the mitochondrial matrix and is well known for its central role for energy production in the Krebs cycle. NADP-dependent IDHs are primarily located either in mitochondria or cytoplasm . Each NADP-dependent isozyme is a homodimer. Mutations of Arg132 of human IDH1 result in a reduced ability of the enzyme to convert isocitrate to alpha ‑ketoglutarate, but the enzyme acquires the ability to generate 2-hydroxyglutarate (2HG) from alpha‑ketoglutarate,2-HG is elevated in several tumor types, including a subset of AMLs.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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