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Your Position: Home > Others > Gastrin-71 > GAN-M5253

Mouse Gastrin-71 Protein, Fc tag

  • Synonym
    Gastrin-71,Gastrin,GAST,Gastrin component
  • Source
    Mouse Gastrin-71 Protein, Fc tag (GAN-M5253) is expressed from human 293 cells (HEK293). It contains AA Ser 22 - Phe 92 (Accession # P48757-1).
    Predicted N-terminus: Ser 22
  • Molecular Characterization
    Online(Ser 22 - Phe 92) P48757-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 34.7 kDa. The protein migrates as 40-45 kDa and 50-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse Gastrin-71 Protein, Fc tag  (Cat. No. GAN-M5253) SDS-PAGE gel

Mouse Gastrin-71 Protein, Fc tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    Gastrin is a peptide hormone that stimulates secretion of gastric acid (HCl) by the parietal cells of the stomach and aids in gastric motility. It is released by G cells in the pyloric antrum of the stomach, duodenum, and the pancreas. Gastrin binds to cholecystokinin B receptors to stimulate the release of histamines in enterochromaffin-like cells, and it induces the insertion of K+/H+ ATPase pumps into the apical membrane of parietal cells (which in turn increases H+ release into the stomach cavity). Its release is stimulated by peptides in the lumen of the stomach.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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