|EP116-C01||High-bind Plate||1 plate|
|EP116-C02||Human CTLA-4||10 μg|
|EP116-C03||Biotinylated Human B7-1||10 μg|
|EP116-C04||Anti-CTLA4 Neutralizing Antibody||20 μg|
|EP116-C06||Coating Buffer||12 mL|
|EP116-C07||10xWashing Buffer||50 mL|
|EP116-C08||Blocking Buffer||50 mL|
|EP116-C09||Substrate Solution||12 mL|
|EP116-C10||Stop Solution||7 mL|
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human CTLA-4.
b) Add your molecule of interest to the tests.
c) Add human B7-1-Biotin to bind the coated human CTLA-4.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to B7-1: CTLA-4 binding will be determined by comparing OD readings among different experimental groups.
B7-1 [BIOTINYLATED]: CTLA-4 BINDING IN THE ABSENCE OF INHIBITORS
Immobilized human CTLA-4 protein at 0.5 μg/mL (100 μL/well) can bind biotinylated human B7-1 with a linear range of 0.0002-0.1 μg/mL when detected by Streptavidin-HRP. Background was subtracted from data points before curve fitting (QC tested).
INHIBITION OF B7-1 [BIOTINYLATED]: CTLA-4 BINDING BY ANTI-CTLA-4 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-CTLA-4 Neutralizing antibody (Catalog # EP116-C04) (1:1 serial dilution, from 10 μg/mL to 0.078μg/mL) was added into CTLA-4: Biotinlated B7-1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Price(USD) : $650.00
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