|EP128-C01||High-bind Plate||1 plate|
|EP128-C02||Human IL-5 R alpha||30 μg|
|EP128-C03||Human IL-5||25 μg|
|EP128-C04||Human IL-5-Biotin||10 μg|
|EP128-C06||Coating Buffer||12 mL|
|EP128-C07||10xWashing Buffer||50 mL|
|EP128-C08||Blocking Buffer||50 mL|
|EP128-C09||Substrate Solution||12 mL|
|EP128-C10||Stop Solution||7 mL|
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-5 R alpha.
b) Add your molecule of interest to the tests.
c) Add human IL-5-Biotin to bind the coated human IL-5 R alpha.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the ability of your compound to inhibit IL-5: IL-5 R alpha Rbinding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF IL-5 [BIOTINYLATED]: IL-5 R ALPHA BINDING BY Human IL-5
Serial dilutions of Human IL-5(Catalog # EP128-C03) (1:1 serial dilution, from 10 μg/mL to 0.020 μg/mL (666.67-0.65 nM)) was added into IL-5 R alpha: IL-5-Biotin binding reactions. The assay was performed according to the above-described protocol. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Price(USD) : $600.00
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