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Your Position: Home > > GM-CSF > CRS-B007

ClinMax™ Human GM-CSF ELISA Kit

For research use only.
Product Details
Assay Type Sandwich-ELISA
Analyte GM-CSF
Format 96T
Reactivity Human
Regulatory Status RUO
Sensitivity <6.0 pg/mL
Standard Curve Range 7.81 pg/mL-1000 pg/mL
Assay Time 2 hr
Suitable Sample Type For the quantitative determination of human GM-CSF in serum, plasma and cell supernatant.
Sample volume 50 μL
Materials Provided
CRB007-C01Pre-coated Anti-GM-CSF Antibody Microplate1 plate
CRB007-C02Human GM-CSF Standard20 μg×2
CRB007-C03Biotin-Anti-GM-CSF Antibody Con. Solution100 μL
CRB007-C04Biotin-Antibody Dilution Buffer8 mL
CRB007-C05Streptavidin-HRP Con. Solution500 μL
CRB007-C06Streptavidin-HRP Dilution Buffer15 mL
CRB007-C0720× Washing Buffer50 mL
CRB007-C081× Dilution Buffer15 mL×2
CRB007-C09Substrate Solution12 mL
CRB007-C10Stop Solution6 mL
  • Background
    Human Macrophage Colony Stimulating Factor 2 (GM-CSF) OneStep ELISA Kit is a one-step quick immunoassay designed to measure human GM-CSF levels in cell culture supernates, serum, and plasma. This assay kit is a standard sandwich-ELISA format. It contains recombinant human GM-CSF and a pair of antibodies against the recombinant factor, which are provided by ACROBiosystems. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of natural and recombinant human GM-CSF concentrations. The specificity has been verified.
  • Application

    The kit is developed for quantitative detection of GM-CSF in human serum and cell culture supernates.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit is used to measure the concentrations of human Interleukin 2 (GM-CSF) by employing a standard sandwich-ELISA format. The microplate in the kit has been pre-coated with Anti- GM-CSF Antibody. First add the standard samples provided in kit and your samples to the plate, next add detection antibody Biotin-Anti-GM-CSF Antibody to the plate, incubate and wash the wells. After wash add HRP-Streptavidin to the plate, incubate and wash the wells. Lastly load the substrate into the wells and color develops in proportion to the amount of Interferon-γ bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450nm and 630nm.
Typical Data Please refer to DS document for The assay protocol.

For each experiment, each ELISA plate needs to set the standard curve. The minimum detectable concentration of CRS-B007 is less than 6.0 pg/mL.

  • Background: GM-CSF
    GM-CSF, Granulocyte Macrophage Colony Stimulating Factor
    GM-CSF is a colony-stimulating factor that regulates growth and differentiation of hematopoietic cells. GM-CSF is a primarily T cell-derived 14-35 kDa variably glycosylated protein which affects growth and functions of granulocytes and cells of the macrophage lineage at all stages of maturation.
    It is also required for the development of specialized alveolar macrophages responsible for pathogen surveillance and the metabolism of pulmonary surfactant. Its production is widespread throughout the body in certain infectious and inflammatory states to enhance a local innate immune response. On the other hand, its abnormal production can lead to chronic inflammatory states as observed in rheumatoid arthritis and asthma. Some of the actions of GM-CSF include enhancing macrophage, neutrophil, eosinophil, and natural killer cell effector functions; dendritic cell survival and differentiation; and the up-regulation of antigen presentation.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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