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| ID | Components | Size |
| EP150-C01 | High-bind Plate | 1 plate |
| EP150-C02 | Human CD28 | 20 μg |
| EP150-C03 | Biotinylated Human B7-1 | 50 μg |
| EP150-C04 | Anti-CD28 Neutralizing Antibody | 20 μg |
| EP150-C05 | Streptavidin-HRP | 5 μg |
| EP150-C06 | Coating Buffer | 12 mL |
| EP150-C07 | 10xWashing Buffer | 50 mL |
| EP150-C08 | Blocking Buffer | 50 mL |
| EP150-C09 | Substrate Solution | 12 mL |
| EP150-C10 | Stop Solution | 7 mL |
This kit is developed for screening for inhibitors of human CD28 binding to human B7-1.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human CD28.
b) Add your molecule of interest to the tests.
c) Add human B7-1-Biotin to bind the coated CD28.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to CD28:B7-1 binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF CD28:B7-1[Biotinylated] BINDING BY ANTI-CD28 NEUTRALIZING ANTIBODY
Serial dilutions of Anti-CD28 Neutralizing antibody (Catalog # EP150-C04) (1:1 serial dilution, from 5 μg/mL to 0.01953125μg/mL) was added into CD28: Biotinylated B7-1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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