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|EP156-C01||High-bind Plate||1 plate|
|EP156-C02||Human IL-12B||15 μg|
|EP156-C03||Biotinylated Human IL-12RB1||30 μg|
|EP156-C04||Anti-IL-12B Neutralizing Antibody||20 μg|
|EP156-C06||Coating Buffer||12 mL|
|EP156-C07||10xWashing Buffer||50 mL|
|EP156-C08||Blocking Buffer||50 mL|
|EP156-C09||Substrate Solution||12 mL|
|EP156-C10||Stop Solution||7 mL|
This kit is developed for screening for inhibitors of human IL-12B binding to human IL-12RB1.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human IL-12B.
b) Add your molecule of interest to the tests.
c) Add human IL-12RB1-Biotin to bind the coated IL-12B.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to IL-12B:IL-12RB1 binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF IL-12B: IL-12RB1[Biotinylated] BINDING BY ANTI-IL-12B NEUTRALIZING ANTIBODY
Serial dilutions of Anti-IL-12B Neutralizing antibody (Catalog # EP156-C04) (1:1 serial dilution, from 10 μg/mL to 0.039μg/mL) was added into IL-12B: Biotinylated IL-12RB1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $650.00
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