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| ID | Components | Size |
| EP153-C01 | High-bind Plate | 1 plate |
| EP153-C02 | Human LIGHT | 25 μg |
| EP153-C03 | Biotinylated Human LTBR | 7 μg |
| EP153-C04 | Anti-LIGHT Neutralizing Antibody | 20 μg |
| EP153-C05 | Streptavidin-HRP | 5 μg |
| EP153-C06 | Coating Buffer | 12 mL |
| EP153-C07 | 10xWashing Buffer | 50 mL |
| EP153-C08 | Blocking Buffer | 50 mL |
| EP153-C09 | Substrate Solution | 12 mL |
| EP153-C10 | Stop Solution | 7 mL |
This kit is developed for screening for inhibitors of human LIGHT binding to human LTBR.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human LIGHT.
b) Add your molecule of interest to the tests.
c) Add human LTBR-Biotin to bind the coated LIGHT.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to LIGHT:LTBR binding will be determined by comparing OD readings among different experimental groups.
INHIBITION OF LIGHT: LTBR[Biotinylated] BINDING BY ANTI-LIGHT NEUTRALIZING ANTIBODY
Serial dilutions of Anti-LIGHT Neutralizing antibody (Catalog # EP153-C04) (1:1 serial dilution, from 10 μg/mL to 0.156μg/mL) was added into LIGHT: Biotinylated LTBR binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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