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Your Position: Home > Kits > PD-L1 > EP-157

Mouse PD-L1 : Mouse PD-1 [Biotinylated] Inhibitor Screening ELISA Kit

For research use only.

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EP157-C01High-bind Plate1 plate
EP157-C02Mouse PD-L125 μg
EP157-C03Biotinylated Mouse PD-130 μg
EP157-C04Anti-Mouse PD-L1 Neutralizing Antibody20 μg
EP157-C05Streptavidin-HRP5 μg
EP157-C06Coating Buffer12 mL
EP157-C0710xWashing Buffer 50 mL
EP157-C08Blocking Buffer50 mL
EP157-C09Substrate Solution12 mL
EP157-C10Stop Solution7 mL
  • Background
    Immune checkpoint pathway is a focal point of today’s cancer research. PD-1 is one of the best characterized checkpoint proteins. The binding between PD-1 and its ligand PD-L1 suppresses T-cell activation and allows cancer cells to escape from body’s immune surveillance. Therefore, the pharmaceutical inhibition of PD-1 or its ligand has been considered a promising strategy by many oncologists.
  • Application

    This kit is developed for screening for inhibitors of Mouse PD-L1 binding to Mouse PD-1.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage
    Unopened kit should be stored at 2°C -8°C upon receiving. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This inhibitor screening ELISA kit is designed to facilitate the identification and characterization of new Mouse PD-L1 pathway inhibitors. The assay takes advantage of our in house-developed binding of biotinylated Mouse PD-1 to immobilized Mouse PD-L1 in a functional ELISA assay, and employs a simple colorimetric ELISA platform. Briefly, we provide you with a Mouse Biotinylated PD-1 protein, a Mouse PD-L1 protein, an anti-Mouse PD-L1 neutralizing antibody (as method verified Std.), and Streptavidin-HRP reagent.

    Your experiment will include 4 simple steps:

    a) Coat the plate with Mouse PD-L1.

    b) Add your molecule of interest to the tests.

    c) Add Mouse PD-1-Biotin to bind the coated Mouse PD-L1.

    d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.

    Finally, the half maximal inhibitory concentration (IC50) of your compound to PD-1: PD-L1 binding will be determined by comparing OD readings among different experimental groups.

Typical Data Please refer to Ds document for the assay protocol.

Serial dilutions of Anti-Mouse PD-L1 Neutralizing antibody (Catalog # EP157-C04) (1:1 serial dilution, from 5 μg/mL to 0.00244μg/mL) was added into Mouse PD-L1: Biotinylated Mouse PD-1 binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).

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