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| ID | Components | Size |
| EP160-C01 | High-bind Plate | 1 plate |
| EP160-C02 | Human CD47 | 35 μg |
| EP160-C03 | Biotinylated Human SIRP gamma | 15 μg |
| EP160-C04 | Anti-CD47 Neutralizing Antibody | 20 μg |
| EP160-C05 | Streptavidin-HRP | 5 μg |
| EP160-C06 | Coating Buffer | 12 mL |
| EP160-C07 | 20xWashing Buffer | 50 mL |
| EP160-C08 | Blocking Buffer | 50 mL |
| EP160-C09 | Substrate Solution | 12 mL |
| EP160-C10 | Stop Solution | 7 mL |
This kit is developed for screening for inhibitors of human CD47 binding to human SIRP alphaV2.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) Coat the plate with human CD47.
b) Add your molecule of interest to the tests.
c) Add human SIRP gamma-Biotin to bind the coated CD47.
d) Add Streptavidin-HRP followed by TMB or other colorimetric HRP substrate.
Finally, the half maximal inhibitory concentration (IC50) of your compound to CD47:SIRP gamma binding will be determined by comparing OD readings among different experimental groups.
CD47: SIRP gamma [Biotinylated] Inhibitor Screening ELISA Kit
Serial dilutions of Anti-CD47 Neutralizing antibody (Catalog # EP160-C04) (1:1 serial dilution, from 5μg/mL to 0.039μg/mL) was added into CD47: SIRP gamma [Biotinylated] binding reactions. The assay was performed according to the protocol described below. Background was subtracted from data points prior to log transformation and curve fitting (QC tested).
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