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Your Position: Home > Kits > ROR1 > CEA-B050

ClinMax™ Human Soluble ROR1 ELISA Kit,PRO

For research use only.

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Product Details
Assay TypeSandwich-ELISA
AnalyteSoluble ROR1
Format96T
ReactivityHuman
Regulatory StatusPRO
Sensitivity0.78125ng/mL
Standard Curve Range0.78125 ng/mL-200 ng/mL
Assay Time2hr
Suitable Sample TypeFor the quantitative determination of human Soluble ROR1 in Cell Culture Supernatants, Plasma, Serum.
Sample volume50 μL
Materials Provided
IDComponentsSize
CEA050-C01Pre-coated Anti-ROR1 Antibody Microplate1 plate
CEA050-C02Human ROR1 Standard20 μg×2
CEA050-C03Biotin-Anti-ROR1 Antibody Con. Solution400 μL
CEA050-C04Biotin-Antibody Dilution Buffer8 mL
CEA050-C05Streptavidin-HRP Con. Solution500 μL
CEA050-C06Streptavidin-HRP Dilution Buffer15 mL
CEA050-C0720× Washing Buffer50 mL
CEA050-C08Sample Dilution Buffer15 mL ×2
CEA050-C09Substrate Solution12 mL
CEA050-C10Stop Solution6 mL
  • Background
    ClinMax™ ELISA Kit is convenient ready-to-use immunoassay Kit, specifically designed to quantitate human ROR1 that is present in complex biological samples, such as human serum, plasma, and cell culture supernates.

    A comprehensive validation of the ELISA method was performed following the ICH M10 on bioanalytical method validation and the FDA’s bioanalytical method validation guidance for industry. This validation included assessments of linearity, accuracy, precision, dilution linearity, recovery, and the hook effect. For details information, please refer to the DS.

    ClinMax™ ELISA Kits are manufactured in a GMP-certified facility and comply to the ISO 13485 standard, ensuring a high level of quality and reliability.

  • Application

    The kit is developed for quantitative detection of human ROR1 in serum, plasma and cell culture supernates.

    It is for research use only.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
  • Assay Principles
     ROR1 Assay Principles
Typical Data Please refer to DS document for the assay protocol.
 ROR1 TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was calculated based on the results of the standard curve. The minimum detectable concentration of ROR1 is less than 0.78125 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of 4 samples containing different ROR1 concentrations were tested in one assay. Acceptable criteria: CV<10%.

 ROR1 INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Five samples containing different concentrations of ROR1 were tested in independent assays. Acceptable criteria: CV<15%.

 ROR1 INTER-ASSAY STATISTICS
Recovery

Recombinant ROR1 was spiked into 3 human serum samples, and then analyzed. The average recovery of ROR1 for serum samples is 103.2%.

 ROR1 RECOVERY
  • Background: ROR1
    Tyrosine-protein kinase transmembrane receptor ROR1 is also known as Neurotrophic tyrosine kinase, receptor-related 1 (NTRKR1), which belongs to the protein kinase superfamily or tyr protein kinase family or ROR subfamily. ROR1 contains 1 FZ (frizzled) domain, 1 Ig-like C2-type (immunoglobulin-like) domain, 1 kringle domain, 1 protein kinase domain. ROR1 is expressed at high levels during early embryonic development.
  • Clinical and Translational Updates

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