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This protein carries no "tag".
The protein has a calculated MW of 35.5 kDa. The protein migrates as 75 kDa±5 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 20 mM NaAc-HAc, pH5.0 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Thrombopoietin Protein, premium grade on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
Human Thrombopoietin Protein, premium grade (Cat. No. THN-H5216) stimulates proliferation of Mo7e cells. The specific activity of Human Thrombopoietin Protein, premium grade is > 1.00ⅹ10^7 IU/mg, which is calibrated against human TPO Standard (NIBSC code: 03/124) (QC tested).
Human Thrombopoietin R, His Tag (Cat. No. THR-H52H7) captured on CM5 chip via anti-His antibody can bind Human Thrombopoietin Protein, premium grade (Cat. No. THN-H5216) with an affinity constant of 2.96 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
The Cell based assay shows batch-to-batch consistency between Acro's GMP and PG Thrombopoietin.
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