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Flow cytometric analysis of HEK293/Human FcRn (FCGRT & B2M) Stable Cell staining with Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F) Protein, His Tag-Cy5 (Cat. No. IG1-HC2H3) at 1:50 dilution(2 μL of the stock solution corresponds to labeling of 2e5 cells in a final volume of 100 µL) , compared with negative control protein. Cy5 signal was used to evaluate the binding activity (QC tested).
2e5 of HEK293/Human FcRn (FCGRT & B2M) Stable Cell Line were stained with 100 μL of 10 μg/mL of FITC-Labeled Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F) Protein, His Tag (Cat. No. IG1-HF2H3) and negative control protein respectively. FITC signal was used to evaluate the binding activity (QC tested).
Human IgG1 Fc Protein, His Tag-Cy5 (IG1-HC2H3) immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) with an affinity constant of 8.27 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Human IgG1 Fc (C103S, M135Y, S137T, T139E, H316K, N317F), His Tag (Cat. No. IG1-H52H8) immobilized on CM4 Chip can bind Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) with an affinity constant of 8.45 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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