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Recombinant ROR1 & ROR2 Proteins

ROR family includes receptor tyrosine kinases ROR1 and ROR2. ROR1 is of particular interest to the pharmaceutical community, because its expression is only observed in embryonic and cancerous cells, but not in health adult tissues. This feature makes it an ideal drug target for several cancer types. Multiple ROR1-targeted therapies are underdevelopment, including Oncternal’s Cirmtuzumab.

ROR1 proteins

The extracellular domain of ROR1 is made of three distinct domains, an immunoglobulin-like (Ig) domain, a frizzled domain, and a kringle domain (Borcherding et al., 2014). Interestingly, antibodies targeting different domains demonstrate a marked difference in therapeutic efficacy when used to treat leukemia cell lines (Daneshmanesh et al., 2013).


ACROBiosystems provides a comprehensive panel of human ROR1 proteins, including product of each single domain (Ig-like, Frizzled, Kringle), and the entire ECD of all three domains. We also have pre-biotinylated ROR1 (ECD). These proteins are generated using Avitag technology. The biotin tag is precisely conjugated on the single Avitag introduced at the C-terminus of the protein. This site-specific labeling method has many advantages over the commonly used NHS-labeling. It not only 100% preserves the protein's natural structure, but also confers uniform labeling efficiency and bioactivity. These pre-labeled products have been used to screen for anti-ROR1 mAbs. Moreover, we also developed mouse and rat ROR1 proteins for animal studies. (see full product list below)

Product Validation Data

BLI verified that the affinity constant of anti-human ROR1 mAb binding to human ROR1 protein was 10.2 nM.

Fig.1 Loaded Anti-Human ROR1 mAb (Mouse IgG1) on AMC Biosensor, can bind Human ROR1, His Tag (Cat. No. RO1-H522y) with an affinity constant of 10.2 nM as determined in BLI assay (ForteBio Octet Red96e).

ELISA verified that the linear range of anti-human ROR1 mAb binding to human ROR1 protein was 0.5-4 ng/mL.

Fig.2 Immobilized Anti-Human ROR1 mAb at 2 μg/mL (100 μL/well) can bind Biotinylated Human ROR1, His,Avitag (Cat. No. RO1-H82E6) with a linear range of 0.5-4 ng/mL.

Product List

ROR1
Cat. No.Product DescriptionStructureDomain
RO1-M5250Mouse ROR1 Protein, Fc Tag 
RO1-M5221Mouse ROR1 Protein, His Tag 
RO1-H82E6
(Biotion-Labeled)
Biotinylated Human ROR1 Protein, His,Avitag™Full ECD
RO1-H82F4
(Biotion-Labeled)
Biotinylated Human ROR1 Protein, Fc,Avitag™Full ECD
RO1-R5221Rat ROR1 Protein, His Tag 
RO1-H821y
(Biotion-Labeled)
Biotinylated Human ROR1 Protein, Avitag™Full ECD
RO1-H5250Human ROR1 Protein, Fc TagFull ECD
RO1-H522yHuman ROR1 Protein, His TagFull ECD
RO1-H5221Human ROR1 (39-151, Ig-like domain) Protein, His TagIg-like
RO1-H5223Human ROR1 (308-395, Kringle domain) Protein, His TagKringle
RO1-H5222Human ROR1 (165-305, Frizzled domain) Protein, His TagFrizzled
ROR2
Cat. No.Product DescriptionStructure
RO2-H82E3
(Biotion-Labeled)
Biotinylated Human ROR2 / NTRKR2 Protein, His,Avitag™
RO2-M5253Mouse ROR2 / NTRKR2 Protein, Fc Tag
RO2-H5251Human ROR2 / NTRKR2 Protein, Fc Tag
RO2-H52E5Human ROR2 / NTRKR2 Protein, His Tag

References

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