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Your Position: Home > Lymphocyte-activation gene 3 (LAG-3)

Lymphocyte-activation gene 3 (LAG-3)

The clinical success of several anti-PD1 and anti-CTLA4 antibodies has boosted the research of other immune checkpoint proteins. The lymphocyte-activation gene 3 (LAG-3), is of particular interest to researchers because it functions differently from the canonical negative checkpoint regulators such as PD1 and CTLA4.

LAG-3 is expressed on cell membranes of natural killer cells (NK), B cells, tumor-infiltrating lymphocytes (TIL), T cells, and dendritic cells (DC), and it appears to play complicated roles in the immune pathway. The binding between soluble LAG-3 (sLAG-3) and MHCII molecules promotes the maturation of dendritic cells, while the interaction between MHCII and LAG-3 on membrane leads to the negative regulation of T-cell activity.


LAG-3 proteins

Several therapeutic agents have been developed to target the LAG-3 pathway. IMP321, a sLAG-3-Fc fusion protein developed by Prima Biomed, is one of the leading drugs that currently being investigated in a phase II clinical trial. IMP321 augments immune response against cancer cells through two distinct mechanisms. It binds with MHCII molecules on the immature dendritic cells to enhance tumor antigen presentation. At the same time, it also blocks the MHCII molecule to bind with LAG-3-expressing CD8+ T-cells, and by so doing prevents the T-cells from being negatively regulated. Other investigative drugs, such as Bristol-Myers Squibb's anti-LAG-3 antibody BMS-986016, directly bind with LAG-3 expressed on the T-cells and block the LAG-3-MHCII interaction. As a result, the T-cells can be activated to boost immune response.


ACROBiosystems provides a comprehensive panel of LAG-3 proteins, including Cynomolgus LAG-3, Mouse IgG2a Fc Tag (Cat. No. LA3-C52A0), Cynomolgus LAG-3, Fc Tag (Cat. No. LA3-C5252), and Human LAG-3, Fc Tag (Cat. No. LA3-H5255).

LAG-3 Product List

Cat. No.SpeciesProduct DescriptionStructurePurityFeatures
LA3-H5222
Human LAG-3 / CD223 Protein, His TagLA3-H5222-STC1.jpg

LA3-H52Aa
Human LAG-3 / CD223 Protein, Mouse IgG2a Fc TagLA3-H52Aa-STC1.jpg

LA3-H82E5
Biotinylated Human LAG-3 / CD223 Protein, His,Avitag™LA3-H82E5-STC1.jpg

LA3-H5255
Human LAG3 / CD223 Protein, Fc Tag (HPLC-verified)LA3-H5255-STC1.jpg

LA3-H82Fb
Biotinylated Human LAG-3 / CD223 Protein, Fc,Avitag™LA3-H82Fb-STC1.jpg

LA3-M52H5
Mouse LAG-3 / CD223 Protein, His TagLA3-M52H5-STC1.jpg

LA3-H82F3
Biotinylated Human LAG-3 / CD223 Protein, Mouse IgG2a Fc,Avitag™LA3-H82F3-STC1.jpg

LA3-C5252
Cynomolgus LAG-3 / CD223 Protein, Fc TagLA3-C5252-STC1.jpg

LA3-C52A0
Cynomolgus LAG-3 / CD223 Protein, Mouse IgG2a Fc TagLA3-C52A0-STC1.jpg

LA3-H525c
Human LAG-3 / CD223 Protein, Llama IgG2b Fc Tag, low endotoxinLA3-H525c-STC1.jpg

LA3-H522a
Human LAG-3 / CD223 Protein, His Tag, low endotoxinLA3-H522a-STC1.jpg

LA3-R52H5
Rat LAG3 / CD223 Protein, His TagLA3-R52H5-STC1.jpg

LA3-M52Ha
Marmoset LAG-3 / CD223 Protein, His TagLA3-M52Ha-STC1.jpg

LA3-HP2H3
PE-Labeled Human LAG-3 / CD223 Protein, His Tag
(recommended for neutralizing assay)
LA3-HP2H3-STC1.jpg
 

Product Highlight

The purity of Human LAG-3 was verified by HPLC.


Fig.1 The purity of Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) was greater than 90% as determined by SEC-HPLC.

The FACS analysis of Human LAG-3 protein against Daudi cells and competitive experiment of neutralizing anti-LAG-3 antibody.

FACS analysis of LAG-3 mFc binding to Daudi cell surface

Fig.2 Flow Cytometry assay shows that Human LAG3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) can bind to Daudi cell surface. The concentration of LAG3 used is 0.3 μg/mL.                    

Competitive experiment of neutralizing anti-LAG-3 antibody

Fig.3 FACS analysis shows that the binding Human LAG3, Mouse IgG2a Fc Tag (Cat. No. LA3-H52Aa) to Daudi cells was inhibited by increasing concentration of neutralizing Anti-LAG3 antibody. The concentration of LAG3 used is 1 μg/mL. The IC50 is 0.165 μg/mL.

BLI verified that the affinity constant of Anti-LAG-3 mAb to Human LAG-3 protein was 7.47 nM.

Fig.4 Loaded Anti-LAG-3 mAb (Human IgG1) on AHC Biosensor, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 7.47 nM as determined in BLI assay (ForteBio Octet Red96e).

The ELISA date showed that the linear range of Anti-LAG-3 mAb to Human LAG-3 protein was 0.4-3 ng/mL and Human LAG-3 protein has high batch-to-batch consistency.

Fig.5 Immobilized Human LAG-3, Fc Tag (HPLC-verified) (Cat. No. LA3-H5255) at 2 μg/mL (100 μL/well) can bind Madarex LAG-3 mAb with a linear range of 0.4-3 ng/mL. The ELISA date showed that the Human LAG-3 protein has high batch-to-batch consistency.

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