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Biotinylated CD3E & more CD3 products

The T-cell receptor (TCR) does not signal on its own. Instead, it is constitutively associated with the CD3 coreceptors, which contain intracellular signaling apparatus, consisting of the CD3εγ and CD3εδ heterodimers and the CD3ζζ homodimer (1,2). CD3 has become a focal point of pharmaceutical research in the last decades. Direct inhibition of CD3 protein has been used to treat autoimmune diseases and allograft rejection. OKT3, the first mouse monoclonal antibody specific for human CD3, was produced in 1979 by P. Kung and G. Goldstein (3). It was also the first monoclonal antibody to be used in clinical practice, in the field of transplantation.

Biotinylated CD3E and more CD3 products

More recently, targeting CD3 has been used as a strategy to bridge cytotoxic T cells to target cells in bi-specific antibody design. A successful example is Amgen’s Blincyto® (blinatumomab). Blinatumomab is a bi-specific antibody simultaneously targeting CD3 and CD19, and by so doing directing the CD3-expressing T cells to CD19-expressing lymphoma cells (4). The capacity of CD3-specific antibody therapy to promote tolerance, a concept that has been proven for autoimmune diabetes, could be extended to other autoimmune diseases and transplantation.

ACROBiosystems provides a comprehensive panel of CD3 proteins, including the CD3 epsilon, CD3 delta, and CD3E & CD3D heterodimer proteins. We also offer the biotinylated CD3 epsilon and the biotinylated CD3E & CD3D heterodimer proteins.

Bioactivity Data

SPR
Fig.1 SP34-2 (Mouse IgG1) captured on CM5 chip via anti-mouse antibodies surface, can bind Human CD3 epsilon, His Tag (Cat. No. CDE-H5223) with an affinity constant of 0.97 nM as determined in a SPR assay (Biacore T200).
Fig.2 SP34-2 (Mouse IgG1) captured on CM5 chip via anti-mouse antibodies surface, can bind Cynomolgus CD3E, His Tag (Cat. No. CDE-C5226) with an affinity constant of 0.981 nM as determined in a SPR assay (Biacore T200).
ELISA
Biotinylated Human CD3E,His Tag ELISA
Fig.3 Immobilized Anti-Human CD3 Mab, mouse IgG1 (Clone # SP34-2) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD3E, His Tag (Cat. No. CDE-H8223) with a linear range of 0.05-2 ng/mL.
Biotinylated Human CD3E and CD3D ELISA
Fig.4 Immobilized Biotinylated Human CD3E & CD3D (Cat. No. CDD-H82W0) can bind OKT3 with a linear range of 2-39 ng/mL. The biotinylated CD3E & CD3D heterodimers are coated at 1 ug/mL (100 μL/well) on a streptavidin coated plates.

CD3 Product List

MoleculeCat. No.SpeciesProduct DescriptionStructurePurityFeatures
CD3 epsilonCDE-H8223
CDE-H8223-STC1Biotinylated Human CD3 epsilon Protein, His Tag, ultra sensitivity (primary amine labeling)

CDE-H5223
CDE-H5223-STC1Human CD3 epsilon Protein, His Tag

CDE-H5256
CDE-H5256-STC1Human CD3 epsilon Protein, His Tag

CDE-H5286
CDE-H5286-STC1Human CD3 epsilon Protein, Strep Tag

CDE-M5256
CDE-M5256-STC1Mouse CD3 epsilon Protein, Fc Tag

CDE-C5226
CDE-C5226-STC1Cynomolgus CD3 epsilon Protein, His Tag

CDE-C5254
CDE-C5254-STC1Cynomolgus CD3 epsilon Protein, Fc,His Tag

CDE-H82E1
CDE-H82E1-STC1Biotinylated Human CD3 epsilon Protein, His, Avitag™

CD3 deltaCDD-H5224
CDD-H5224-STC1Human CD3 delta Protein, His Tag

CDD-C52H8
CDD-C52H8-STC1Cynomolgus CD3 delta Protein, His Tag

CD3E & CD3DCDD-H82W0
CDD-H82W0-STC1Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag, ultra sensitivity (primary amine labeling)

CDD-H52W4
CDD-H52W4-STC1Human CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free

CDD-H52W0
CDD-H52W0-STC1Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag

CDD-C52W4
CDD-C52W4-STC1Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag

CDD-M5219
CDD-M5219-STC1Mouse CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag

CDD-C82W6
CDD-C82W6-STC1Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag™&Tag Free

CDD-H5258
CDD-H5258-STC1Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin

CDD-C5259
CDD-C5259-STC1Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin

CDD-H82W1
CDD-H82W1-STC1Biotinylated Human CD3 epsilon&CD3 delta Heterodimer Protein, His,Avitag™&Tag Free

CD3E & CD3GCDG-H52W9
CDG-H52W9-STC1Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag

CDG-C52W6
CDG-C52W6-STC1Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free  
CDG-C5255
CDG-C5255-STC1Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag

References

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