Streptavidin(SA) Series Products

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ACROBiosystems

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SA- Biotin Systems

In 1963, Stapley EO first isolated streptavidin (SA) from the culture medium of Streptomyces avidinii. The binding between SA and biotin is one of the strongest non-covalent interactions known in nature. SA and biotin bind with high affinity producing a significant amplification effect, making the SA-biotin system an asset that is widely utilized in biological and chemical experiments.

SA-biotin systems are often coupled to antibodies or proteins, where SA is conjugated to labels such as HRP (Horseradish Peroxidase), fluorophores, etc., and biotin is coupled to antibodies or proteins (MABSOL® Biotinylated Proteins). The SA-biotin system is widely used in the qualitative and quantitative detection of trace antigens, antibodies and localization observation research to achieve indirect detection of targets, making immunolabeling and related tracer analysis more sensitive.

ACROBiosystem’s SA series of products includes recombinant SA protein, labeled SA protein, SA coated plates, SA magnetic beads, etc., to accelerate your scientific research projects and drug development.

SA Series Products

Molecule	Product Category	Cat. No.	Product Description	Preorder/Order
Streptavidin	SA	STN-N5116	Recombinant Streptavidin	Order
	Labeled SA	STN-NH913	Streptavidin-HRP, Horseradish peroxidase conjugated Streptavidin	Order
		STN-NA114	Streptavidin-Acridine ester (MALS verified)	Order
		STN-NA118	Streptavidin-Alexa Fluor 488 (MALS verified)	Order
		STN-NA115	Streptavidin-Alexa Fluor 555 (HPLC verified)	Order
		STN-NA119	Streptavidin-Alexa Fluor 647 (HPLC verified)	Order
	SA Coated Plates	SP-11	Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame	Order
		SP-13	Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (For Serological Testing)	Order
		SP-14	Streptavidin Coated Plates, Clear, 96-Well, Clear Frame	Order
		SP-15	Streptavidin Coated Plates, Clear, 96-Well, Clear Frame (For Serological Testing)	Order
	SA Magnetic Beads	SMB-B01	Magnetic Beads™ Streptavidin	Order

Verification Data

Application data for SA(ELISA)

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD19 Antibody, Mouse IgG2a with a linear range of 0.1-6 ng/mL (QC tested).

Protocol

Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (Cat. No. SPD-C82E9) at 1 μg/mL (100 μL/well) on Streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate, can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

Protocol

Application data for SA coated plate

Immobilized Biotinylated Human CD19 (20-291), His,Avitag (Cat. No. CD9-H82E9) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Anti-FMC63 antibody with a linear range of 0.1-3 ng/mL (QC tested).

Protocol

Immobilized Biotinylated Human PD-1, Fc,Avitag,His Tag (Cat. No. PD1-H82F4) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Human PD-L1, mFc Tag with a linear range of 0.4-6.25 ng/mL (QC tested).

Protocol

Immobilized Biotinylated Human CD20 Full Length, His,Avitag (HEK293) (Cat. No. CD0-H82E5) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Rituximab with a linear range of 78-625 ng/mL (QC tested).

Protocol

Immobilized Biotinylated Human FCGRT&B2M Heterodimer Protein, His,Avitag (Cat. No. FCM-H82W7) at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind Ipilimumab with a linear range of 78-1250 ng/mL (QC tested).

Protocol

Application data for SA magnetic beads

Immobilized 40 μg SARS-CoV-2 S1 protein to 1mg Beads （Cat. No. SMB-B01） can bind the Anti-SARS-CoV-S protein RBD Antibody with an EC50 1.157 μg/mL (QC tested).

Protocol

MABSOL® Biotinylated Proteins

Reference

Louis Chaiet, Frank J. Wolf. The properties of streptavidin, a biotin-binding protein produced by Streptomycetes, Archives of Biochemistry and Biophysics (1964). https://doi.org/10.1016/0003-9861(64)90150-X.