Acrobiosystems for English
icon_bulk_orderBulk inquiry/Quick order
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Insights > Useful tools for T cell culture
Useful tools for T cell culture
Release time: 2022-07-26 Source: ACROBiosystems Read: 3406

T cell activation plays a central role in the immune response. Its activation, proliferation and differentiation require dual stimulation signals. The first signal is the combination of TCR/CD3 and antigen-presenting cell (APCs) surface-specific MHC molecule antigen-peptide complexes. The second signal is a non-specific co-stimulatory signal, which is generated by the interaction of multiple pairs of co-stimulatory molecules on the surface of APCs and the corresponding receptors of T cells (such as: CD28, CTLA-4 and CD80, CD86, 4-1BB and 4-1BBL, CD40 and CD40L, PD-1 and PD-L1, etc.), among which CD28 is the most important costimulatory molecule, and the second signal can fully activate T cells, secrete cytokines and expresses cytokine receptors. Studies have shown that the in vitro activation and proliferation of T cells plays an important role in research fields such as cell therapy and immune checkpoints.

Cell therapy

CAR-T and TCR-T therapy are the current research hotspots of adoptive immune cell therapy. Whether it is CAR-T or TCR-T cell culture in vitro, T cells need to be activated before subsequent operations can be performed. Therefore, a suitable T cell activation reagent is an essential raw material for the development of cell therapy drugs. At present, the main methods of activation of T cells in vitro on the market are: ① Use soluble antibodies, such as Anti CD3/CD28 antibodies, combined with cytokines such as IL-2 for stimulation; ② Using antibodies bound to solid-phase carriers, such as CD3/CD28 antibody-coupled magnetic beads, and the appropriate T cell activation method can be selected based on the activation strength and subsequent residual detection considerations.

Activation of T cells during cell therapy development

Figure 1 Activation of T cells during cell therapy development

Immune checkpoint research

Immune checkpoint drug have become the focus of attention in tumor immunotherapy due to their excellent clinical efficacy, particularly antibody targeting immune checkpoints, a hot spot in immunotherapy, have become the forefront of competition among pharmaceutical giants. The key points of antibody development are not only functional effects, but activity assays in vitro are also important, which can provide early data support for functional efficacy in vivo. At present, the in vitro activity research methods for immune checkpoint antibody mainly focus on the activation activity of T cells. For example, CD3 antibodies can be coated on PBMC culture plates, and CD28 soluble antibodies can be added to the supernatant to activate T cells, or by CD3 /CD28 antibody coupled with magnetic beads to activate, and then the content of cytokines in the culture supernatant containing the immune checkpoint drug was detected to reflect the activation of the drug on T cells.

Immune checkpoint drugs killing tumor cells

Figure 2 Immune checkpoint drugs killing tumor cells

Recommended Products

5.5 μm size, can better simulate APC and stimulate T cells

Strong magnetism, easy separation, magnetic beads are not easy to remain

Ultra low endotoxin (< 2EU/mg), no damage to T cells

Verified by cell-based assay, it can efficiently activate and expand T cells


Low matrix interference tested by cells culture medium and real samples of serum

Kit standards are calibrated by WHO standard NIBSC/WHO (87/586)

Both RUO and ClinMax version are available to meet the needs of R&D and clinical trials

Strict control of sensitivity, specificity, accuracy, linearity and inter-/intra-assay precision

High purity verified by MALS or HPLC

High bioactivity verified by ELISA/SPR/FACS, etc.

High batch to batch consistency by strict QC tested

T cell culture

T cells are fully activated by the first and second signal stimulation, and they also depend on a variety of cytokines (IL-1, IL-2, IL-4, IL-6, IL-10, IL-12, IL-15 and IFN-γ, etc.) for further proliferate and activate. Otherwise, activated T cells cannot proliferate and differentiate, resulting in apoptosis. Therefore, media supplements such as IL-15, IL-7, and IL-21 and other cytokines are important reagents for the proliferation and differentiation of T\NK immune cells. Both FDA and CDE have relevant regulations on the use of these key materials. The FDA, CMC recommends using FDA-approved or clinical-grade materials. For Chinese Pharmacopoeia regulations, priority should be given to the use of low-risk materials such as GMP grade materials as opposed to non-GMP grade materials. Therefore, safe, effective, and compliant cytokines are crucial for the success of R&D processes and applications of immune cell therapy drugs.

The development of CAR-T requires cytokines and other culture materials

Figure 3 The development of CAR-T requires cytokines and other culture materials

Recommended Products

Strict quality control standards

Excellent safety profile (tested for sterility, mycoplasma, endotoxin, and residual impurities)

High stability and batch-to-batch consistency

Comprehensive regulatory support files


As an important immune cell, T cells play a key role in the development of immune cell therapy culture. Faced with a variety of T cell culture-related reagents on the market, selecting safe and compliant products is crucial for drug development and regulatory approval. To meet market requirements, ACROBiosystems has developed a series of related products for T cell activation and culture that have been released under strict quality standards to accelerate drug development process.

Product List
Anti-CD3/CD28 Ab-coupled Magnetic Beads
(recommended for human T cell activation & expansion)
Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed 2.5 mg2.5 × 107 beads
10 mg1 × 108 beads

Request for Free Sample

GMP-grade Cytokines
MoleculeCat.No.Product descriptionPreorder/Order
IL-15GMP-L15H13GMP Human IL-15


IL-7GMP-L07H24GMP Human IL-7


IL-21GMP-L21H25GMP Human IL-21


Request for Free Sample

Cytokine ELISA Kit
MoleculeCat. No.Product DescriptionPreorder/Order
IFN-γCRS-A001Human Interferon-γ(IFN-γ) ELISA Kit


IL-2CRS-A003Human Interleukin-2(IL-2) ELISA Kit coming soon


IL-4CRS-A004Human Interleukin-4(IL-4) ELISA Kit coming soon


IL-6CRS-A005Human Interleukin-6(IL-6) ELISA Kit coming soon


IL-8CRS-A007Human Interleukin-8(IL-8) ELISA Kit coming soon


IL-10CRS-A008Human Interleukin-10(IL-10) ELISA Kit coming soon


IL-1betaCRS-A012Human Interleukin-1beta(IL-1beta) ELISA Kit coming soon


GM-CSFCRS-A013Human Macrophage Colony Stimulating Factor 2(GM-CSF) ELISA Kit coming soon


Learn more

Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed can effectively expand T cells and have a higher expansion ability than that of other competitors

The purified human T cells were stimulated using Human T cell Activation/Expansion CD3/CD28 Beads, premium grade DMF Filed (Cat. No. MBS-C001) and competitor’s beads respectively. Cells were expanded in T cell culture medium supplemented with 4ng/mL of rhIL-2 Protein (Acrobiosystems, Cat. No. IL2-H4113). Activated Cells were expanded for up to 13 days (A) with high cell viability (B).

GMP Human IL-15 (GMP-L15H13) has high batch-to-batch consistency
Discovery Solutions for Cell and Gene Therapy


[1] Kay, J.E., 1991. Mechanisms of T lymphocyteactivation. Immunology Letters 29, 51 – 54

[2] Trickett A, Kwan YL. T cellstimulation and expansion using anti-CD3/CD28 beads. J Immunol Methods. 2003Apr 1;275(1-2):251-5.

[3] Kalos M, Levine BL, Porter DL, KatzS, Grupp SA, Bagg A, June CH: T cells with chimeric antigen receptors havepotent antitumor effects and can establish memory in patients with advancedleukemia. Sci Transl Med 2011, 3:95ra73.

[4] Hollyman D, Stefanski J, PrzybylowskiM, Bartido S, Borquez- Ojeada O, Taylor C, Yeh R, Capacio V, Olszewska M, HoseyJ et al.: Manufacturing validation of biologically functional T cells targetedto CD19 antigen for autologous adoptive cell therapy. J Immunother 2009, 32:169-180.


This web search service is supported by Google Inc.

Call us
Call us
North America:
+1 800-810-0816 (Toll Free)
Asia & Pacific:
+86 400-682-2521
+1 888-377-6111
1 Innovation Way, Newark, DE 19711, USA

Leave a message