Till April, 2021, according to the data published on ClinicalTrials.gov, there are 1,358 active cell therapy trials, among which most trials are focusing on haematological malignancies, Typical targets include CD19, BCMA and CD22.
ACROBiosystems offers a series of CAR-T target proteins for haematological tumor, such as CD19, BCMA, CD20, CD30 products, which are suitable for detecting CAR expression in clinical trials.
1e6 of the anti-CD19 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human CD19 (20-291), His Tag (Cat. No. CD9-HP2H3). PE Streptavidin was used as negative control.
293 cells were transfected with anti-CD22-scFv and RFP tag. 2e5 of the cells were stained with FITC-Labeled Human Siglec-2, His Tag (Cat. No. SI2-HF2H6, 10 µg/mL) and FITC-labeled Protein Control. Non-transfected 293 cells and FITC-labeled Protein Control were used as negative control. RFP was used to evaluate CAR (anti-CD22-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human Siglec-2, His Tag (Cat. No. SI2-HF2H6).