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Your Position: Home > B7-CD28 family: significant co-stimulatory pathway in immune regulation

B7-CD28 family: significant co-stimulatory pathway in immune regulation

Background

Based on the recognition of antigens in the adaptive immune response, T cell activity is largely achieved during activation, where two signals are required. First, T cell receptors must specifically engage peptides presented by major histocompatibility complexes (MHCs) on antigen presenting cells (APCs); secondly, co-stimulatory/ co-inhibitory receptors on T cells must bind B7 ligand family expressed on APCs to regulate situation of T cell.

This provides a key checkpoint in the regulation of T cell immunity. T cell activation is regulated by the innate immune system through positive and negative costimulatory molecules. Members of the B7 family have been shown to be important for regulating T cell responses. So B7 ligand family plays a significance role in human cancers and autoimmune diseases. The B7 ligand family comprises 11 members, which are B7-1, B7-2, B7-H1, B7-DC, B7-H2, B7-H3, B7-H4, B7-H5, BTNL2, B7-H6 and B7-H7.

ACROBiosystems has developed exclusive B7 family proteins with high purity and high bioactivity, which are suitable for immune, antibody screening, SPR, cell activity detection and other experiments, and can accelerate the development of antibody drugs.

B7-H3
                   
B7-DC
                   
B7-H1
                   
B7-1
                   
B7-2
MHC-11
B7-H2
                   
B7-H4
                   
B7-H5
                   
B7-H7
BTNK-2
B7-H6
TLT-2
PD-1
                               
B7-H1
                   
CD28
                   
CTLA-4
                   
CD3
                       
CD3
                   
ICOS
                   
VISTA
                   
TMIGD2
                   
NKp30
APC
T Cell
ITAM
Actived T Cell
NKC
Second Signal
Second Signal
TCR
First Signal

Stimulatory signal  Inhibitory signal

Arrows indicate interaction between receptor and ligand of B7 family members

Click the molecule name to view product details

Sketch of B7 family molecules
B7 family ligandLigand aliasExpressionPutative receptorsRole in T/NK cell responses
Immune cellsTumor
B7-H1PD-L1,CD274,PDCD1LG1++PD-1,CD80Inhibition
B7-DCPD-L2,CD273,PDCD1LG2++PD-1,RGMBInhibition
B7-1 CD80,BB1,LAB-7,CD28LG1+
CD28,CTLA-4, PD-L1Activation/Inhibition
B7-2 CD86,B70,LAB72,CD28LG2+
CD28,CTLA-4Activation/Inhibition
B7-H3 CD276,B7RP-2++UnidentifiedActivation/Inhibition
B7-H5VISTA,GI24,VSIR,SISP1,PD1H,DD1α+-VSIG3Inhibition
B7-H2ICOS-L,ICOSLG,GL50,B7RP1,LICOS+
ICOS,CD28,CTLA-4Activation
B7-H4B7x,B7S1,B7h.5,VTCN1++UnidentifiedInhibition
B7-H6NCR3LG1,NR3L1-+NKp30Activation
B7-H7HHLA2,B7y++CD28HActivation/Inhibition
Product list
  • Inhibition

  • Activation

  • Activation/Inhibition

MoleculeCat.No.HostProduct descriptionStructure
MoleculeCat.No.HostProduct descriptionStructure
MoleculeCat.No.HostProduct descriptionStructure
Data assay

High purity verified by MALS


           

The purity of Human B7-H3, His Tag (Cat.No. B73-H52E2) was more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

High batch to batch consistency

Designed for different applications of drug development

Application 1: Development and screening of antibody drugs

Immobilized Human ICOS, Fc Tag (Cat. No. ICS-H5258) at 1 μg/mL (100 μL/well) can bind Human B7-H2, His Tag (Cat. No. B72-H5221) with a linear range of 5-78 ng/mL (QC tested).             
 

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag (Cat. No. CT4-H5255): Biotinylated Human B7-1, Fc,Avitag (Cat. No. B71-H82F2) binding reactions. The half maximal inhibitory concentration (IC50) is 0.8260 μg/mL (Routinely tested).

Application 2: Affinity detection of antibody drugs

Anti-Human PD-L1 Mab (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-L1, His Tag (Cat.No. PD1-H5229) with an affinity constant of 0.286 nM as determined in a SPR assay (Biacore T200)(Routinely tested).

Application 3: Cell-based assay

Flow Cytometry assay shows that Biotinylated Human B7-1, Fc,Avitag (Cat. No. B71-H82F2) can bind to 293 cell overexpressing human CTLA-4. The concentration of Human B7-1 is 0.3 μg/mL (Routinely tested).
 
 

FACS analysis shows that the binding of Biotinylated Human B7-1, Fc,Avitag (Cat. No. B71-H82F2) to 293 overexpressing CTLA-4 was inhibited by increasing concentration of neutralizing Anti-Human CTLA-4 antibody. The concentration of B7-1 used is 0.3 μg/mL. The IC50 is 3.025 μg/mL (Routinely tested).

Human B7-H3 (4Ig), His Tag (Cat. No. B7B-H52E7) inhibits Anti-CD3-induced proliferation of PBMC. The EC50 for this effect is 7.95 μg/mL (Routinely tested).

Immobilized cell surface PD-1 (5x104 of cells per well) can bind Human PD-L1, Fc Tag (Cat. No. PD1-H5258) with an EC50 of 0.029 μg/mL (Routinely tested).

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