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As the bispecific antibody (bsAb) technology platform gradually develops and matures, the exploration of target combination mechanisms has become one of the core aspects of future competition. Current bsAb projects are focused on combinations of tumor surface targets and CD3, which is expressed on the surface of T cells, helping mobilize T cells to target tumor cells.

ACROBiosystems provides a series of high-quality CD3 proteins with multiple subtypes, tags, and species to assist in bsAb drug development.

Product List

CD3E & CD3D
CD3E & CD3G
CD3 epsilon
CD3 delta
CD3 gamma

Molecule	Cat. No.	Host	Product Description
CD3E & CD3D	CDD-H5255	HEK293	Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)^New
	CDD-H52Wa	HEK293	Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)^Hot
	CDD-H82W6	HEK293	Biotinylated Human CD3 epsilon&CD3 delta Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)^Hot
	CDD-H52W1	HEK293	Human CD3 epsilon & CD3 delta Heterodimer Protein, His Tag&Tag Free (MALS verified)^Hot
	CDD-H5258	HEK293	Human CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin
	CDD-H82F6	HEK293	Biotinylated Human CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His,Avitag™&Fc,Flag,Avitag™ (MALS verified)
	CDD-H82W0	HEK293	Biotinylated Human CD3E&CD3D Heterodimer Protein, Fc,His Tag&Fc,Flag Tag, ultra sensitivity (primary amine labeling) (MALS verified)
	CDD-C5259	HEK293	Cynomolgus CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin
	CDD-C52W4	HEK293	Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)
	CDD-C52W9	HEK293	Cynomolgus CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)
	CDD-C82W6	HEK293	Biotinylated Cynomolgus CD3E&CD3D Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)
	CDD-M5219	HEK293	Mouse CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)
	CDD-M52W2	HEK293	Mouse CD3 epsilon&CD3 delta Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)
	CDD-M82W5	HEK293	Biotinylated Mouse CD3 epsilon&CD3 delta Heterodimer Protein, His,Avitag™&Tag Free
	CDD-R5257	HEK293	Rat CD3 epsilon&CD3 delta Heterodimer Protein, Llama Fc Tag&Llama Fc Tag (MALS verified)
	CDD-R52D8	HEK293	Rat CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)
	CDD-R5259	HEK293	Rabbit CD3E&CD3D Heterodimer Protein, Llama Fc&Llama Fc, low endotoxin (MALS verified)
	CDD-R52D7	HEK293	Rabbit CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)
	MBS-K003		Human CD3E & CD3D Heterodimer-coupled Magnetic Beads

Molecule	Cat. No.	Host	Product Description
CD3E & CD3G	CDG-H5253	HEK293	Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)^New
	CDG-H52W9	HEK293	Human CD3 epsilon&CD3 gamma Heterodimer Protein, Fc,His Tag&Fc,Flag Tag (MALS verified)^Hot
	CDG-H52W5	HEK293	Human CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Tag Free (MALS verified)
	CDG-H82W3	HEK293	Biotinylated Human CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)
	CDG-C5257	HEK293	Cynomolgus CD3E&CD3G Heterodimer Protein, Fc Tag&Fc Tag (MALS verified)
	CDG-C52W6	HEK293	Cynomolgus CD3E&CD3G Heterodimer Protein, His Tag&Tag Free (MALS verified)
	CDG-C8219	HEK293	Biotinylated Cynomolgus CD3E&CD3G Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)
	CDG-M52D2	HEK293	Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag (MALS verified)
	CDG-M52W2	HEK293	Mouse CD3 epsilon&CD3 gamma Heterodimer Protein,Fc,His Tag&Fc,Flag Tag (MALS verified)
	CDG-M58D2	HEK293	Biotinylated Mouse CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)
	CDG-R82D4	HEK293	Biotinylated Rat CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)
	CDG-R82D3	HEK293	Biotinylated Rabbit CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Flag Tag (MALS verified)
	CDG-RB52D5	HEK293	Rabbit CD3 epsilon&CD3 gamma Heterodimer Protein, His Tag&Flag Tag (MALS verified)

Molecule	Cat. No.	Host	Product Description
CD3 epsilon	CDE-M525a	HEK293	Biotinylated Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a,Avitag™(Clone: OKT3), Ultra-low endotoxin (MALS verified)
	CDE-H5223	HEK293	Human CD3 epsilon Protein, His Tag (MALS verified)^Hot
	CDE-H5256	HEK293	Human CD3 epsilon Protein, Fc,His Tag^Hot
	CDE-C5226	HEK293	Cynomolgus CD3 epsilon Protein, His Tag (MALS verified)^Hot
	CDE-H8224	HEK293	Biotinylated Human CD3 epsilon Protein, His Tag, ultra sensitivity (primary amine labeling)
	CDE-H82E1	HEK293	Biotinylated Human CD3 epsilon Protein, His,Avitag™
	CDE-HR2H4	HEK293	HRP-Human CD3 epsilon Protein, His Tag
	CDN-H5253	HEK293	Human CD3 epsilon Protein, Mouse IgG2a Fc Tag (MALS verified)
	CDE-C5254	HEK293	Cynomolgus CD3 epsilon Protein, Fc,His Tag
	CDE-M5256	HEK293	Mouse CD3 epsilon Protein, Fc Tag
	MBS-K026		Human CD3E-coupled Magnetic Beads

Molecule	Cat. No.	Host	Product Description	Structure
CD3 delta	CDD-H5224	HEK293	Human CD3 delta Protein, His Tag
CD3 delta	CDD-C52H8	HEK293	Cynomolgus CD3 delta Protein, His Tag

Molecule	Cat. No.	Host	Product Description	Structure
CD3 gamma	CDA-H5254	HEK293	Human CD3 gamma / CD3G Protein, Fc Tag (MALS verified)

Questions & Answers

Q1: Because the molecular weight of the Fc on the two isoforms of CDD-H5255 (Human CD3 epsilon and CD3 delta heterodimer protein, Fc Tag & Fc Tag) are so similar, how can you confirm that CDD-H5255 is a real heterodimer?

A1: We have specially designed this product to ensure that CD3E and CD3D form a heterodimer which is verified through two different aspects: structure and activity. The structure is verified through SEC-MALS and reducing / non-reducing electrophoresis. On the other hand, activity is verified through binding activity to our CD3 antibody, OKT3. OKT3 only recognizes CD3 epsilon in CD3E&CD3D, therefore confirming that CD-H5255 is a real, active heterodimer.

CD3E&CD3D (Cat. No. CDD-H5255) is 1:1 heterodimer as verified by non-reducing electrophoresis and MALS.

CD3E&CD3D (Cat. No. CDD-H5255) can specifically bind to Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), as verified by ELISA.

Q2: Have you tested the affinity between CDE-H5223 (Human CD3 epsilon Protein, His tag) protein and Anti-CD3 antibody (UCHT1)?

A2: No, we have not tested the affinity of UCHT1 and CD3 epsilon (Cat. No. CDE-H5223) protein by SPR and BLI methods. However, previous literatures have identified that UCHT1 cannot bind to CD3 epsilon resulting in no affinity. Please refer to the corresponding literature to learn more.

>> A conformational epitope expressed upon association of CD3-epsilon with either CD3-delta or CD3-gamma is the main target for recognition by anti-CD3 monoclonal antibodies.

Q3: I found that some CD3 products are listed as offline. What does this mean?

A3: Our offline products are products that have been updated with newer, updated products. Our newer products have been further optimized to improve factors such as purity and activity. For example, some of our offline products include CDE-H8223, CDD-H82W1, and CDD-H52W4. Online versions of these products are CDE-H8224 (MALS verified heterodimer purity> 90%), CDD-H82W6 (MALS-verified heterodimer purity>85%), CDD-H52W1 (MALS-verified heterodimer>85%; better binding activity to OKT3) respectively. If you cannot find the corresponding online product to your offline one, please feel free to contact us.

Q4: Is the CD3 protein lyophilized? Has the protein been functionally tested after lyophilization?

A4: Yes, our CD3 proteins are lyophilized. Functional testing of our proteins is performed before and after lyophilization.

Different lyophilized batches of Human CD3 epsilon Protein, His Tag (MALS verified) (Cat. No. CDE-H5223) have the same biological activity as the product before lyophilization, with high consistency and verified by ELISA.

Q5: Does CD3 proteins contain BSA?

A5: No. We use trehalose as a protective agent to exclude animal-derived interferences.

Q6: I want to confirm if the Fc tag in CD3E is human IgG1 or mouse IgG1?

A6: The Fc tag is human IgG1. If the species of the Fc tag is unlisted, it can be assumed that our Fc-tagged proteins carry human IgG Fc-tag.

Q7: When selecting and using Fc-tagged CD3E&CD3D and CD3E&CD3G heterodimeric proteins for establishing antibody binding verification methods, what are some potential issues that should be considered when selecting chromogenic secondary antibodies?

A7: One issue to take into consideration is the cross-reactivity between anti-human IgG Fc antibody / llama IgG Fc tags, and anti-llama IgG Fc antibody / human IgG Fc tag. When using the Fc-tagged CD3 antigen to verify antibody binding activity, careful selection of the chromogenic secondary antibody is critical to avoid cross-reactivity.

For more information, please Ask Us Anything About Our CD3 Products