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Llama IgG Fc Fusion Proteins - uniquely designed for llama immunization

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The single domain antibody was originally discovered in camels back in 1993. This new class of antibody is heavy chain only, containing a single variable domain (VHH) and two constant domains (CH2, CH3). This rare form of antibody has following unique advantages, which makes it an intriguing alternative to the conventional antibodies when it comes to therapeutic use.

First, because of its small size, these nanobodies are known to be able to reach to the cryptic epitopes of the targets normally not accessible by the much larger traditional antibodies. The stability of these antibodies is much better than the traditional antibodies, which makes it possible to be delivered through oral and maybe ocular routes, in addition to intravenous and subcutaneous injection. The nanobodies can also be easily conjugated with cell-killing agents if needed. Last but not least, from manufacturing standpoint, nanobodies are much easier to produce from a variety of hosts.

In October 2017, Belgium-based pharmaceutical company Ablynx reported positive results for its first-in-class nanobody drug caplacizumab from a phase III trial on acquired thrombotic thrombocytopenic purpura (aTTP) patients. This success clearly proves the potential for nanobody drugs.

The general process for producing VHH

ACROBiosystems provides a unique portfolio of llama lgG Fc tagged recombinant antigens, which are uniquely designed for llama immunization.

Key Features

  • The llama IgG Fc can promote protein dimerization to enhance immunogenicity

  • The tag has low immunogenicity

  • Long half-life and good stability

  • High purity (Fig 1)

  • High activity (Fig 2)

  • Low endotoxin (less than 10 EU/mg)

Performance of proteins conjugated with llama IgG Fc tag

Human PD-1, Llama IgG2b Fc Tag

Fig. 1 Human PD-1, Llama IgG2b Fc Tag, low endotoxin (Cat. No. PD1-H5259) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.

Immobilized Human BAFF

Fig. 2 Immobilized Human BAFF, His Tag (Cat. No. BAF-H5248) at 5 μg/mL (100 μL/well) can bind Human BCMA, Llama IgG2b Fc Tag (Cat. No. BCA-H5259) with a linear range of 0.02-0.4 ng/mL.

Product list of proteins conjugated with llama IgG Fc tag (Exclusive)

Molecule Cat. No. Product Description Structure
BCMA BCA-H5259 Human BCMA, Llama IgG2b Fc Tag, low endotoxin
CD19 CD9-H5250 Human CD19 (20-291), Llama IgG2b Fc Tag, low endotoxin
CD30 TN8-H5250 Human CD30, Llama IgG2b Fc Tag, low endotoxin
CD38 CD8-H5252 Human CD38, Llama IgG2b Fc Tag, low endotoxin
CD47 CD7-H5251 Human CD47, Llama IgG2b Fc Tag, low endotoxin
LAG-3 LA3-H525c Human LAG-3, Llama IgG2b Fc Tag, low endotoxin
PD-1 PD1-H5259 Human PD-1, Llama IgG2b Fc Tag, low endotoxin
PD-L1 PDL-H5250 Human PD-L1, Llama IgG2b Fc Tag, low endotoxin
Siglec-2 SI2-H525a Human Siglec-2, Llama IgG2b Fc Tag, low endotoxin
Siglec-3 CD3-H5259 Human Siglec-3, Llama IgG2b Fc Tag, low endotoxin

For subsequent biopanning of phage display library, we recommend the corresponding biotinylated protein, which can be coated on Streptavidin-covered surface, for rapid high-throughput screening.

Product list of recommended biotinylated proteins

Molecule Cat. No. Product Description Structure
BCMA BC7-H82F0 Biotinylated Human BCMA / TNFRSF17 Protein, Fc Tag, Avi Tag (Avitag™)
CD30 CD0-H82E6 Biotinylated Human CD30 / TNFRSF8 Protein, Avi Tag (Avitag™)
CD38 CD8-H82E7 Biotinylated Human CD38 Protein, Avi Tag (Avitag™)
CD47 CD7-H82E9 Biotinylated Human CD47 Protein, Avi Tag (Avitag™)
LAG-3 LA3-H82F3 Biotinylated Human LAG-3, Mouse IgG2a Fc Tag, (Avitag™)
PD-1 PD1-H82E4 Biotinylated Human PD-1(recommended for biopanning)
PD-L1 PD1-H82E5 Biotinylated Human PD-L1 / B7-H1 (recommended for biopanning), Avi Tag (Avitag™)
Siglec-2 SI2-H82F8 Biotinylated Human Siglec-2, Fc Tag, (Avitag™)
Siglec-3 CD3-H82E7 Biotinylated Human Siglec-3 / CD33 Protein, Avi Tag (Avitag™)

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